大鼠RAGE ELISA试剂盒(ab100780)

概述

  • 产品名称大鼠RAGE ELISA试剂盒
    参阅全部 RAGE 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 300 pg/ml
  • 范围
    0.41 ng/ml - 300 ng/ml
  • 复检

    72 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 79.4 70% - 91%
    Plasma 74.84 69% - 88%
    Cell culture media 64.74 53% - 70%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s RAGE Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat RAGE in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Rat RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • 组织特异性Endothelial cells.
  • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • Advanced glycosylation end product-specific receptor
    • Ager
    • MGC2235
    • RAGE_HUMAN
    • Receptor for advanced glycosylation end products
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat RAGE ELISA Kit 图像

  • Representative standard curve using ab100780

实验方案

Rat RAGE ELISA Kit (ab100780)参考文献

ab100780 has not yet been referenced specifically in any publications.

Product Wall

The laboratory has informed me that ab100780 will detect soluble RAGE.

Thank you for contacting us.


Although this particular kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test th...

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Thank you for calling Abcam yesterday.

I have talked to the lab and we should be able to add the standard from ab100780 to the catalogue. It may take a week or so though. I will contact you with the abID when it is ready to be ordered.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"