大鼠Prolactin R ELISA试剂盒(PRLR) (ab113351)

概述

  • 产品名称大鼠Prolactin R ELISA试剂盒(PRLR)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.4 ng/ml
  • 范围
    0.512 ng/ml - 50 ng/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 125.4 117% - 141%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Prolactin R Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Prolactin R in cell culture supernatants.

    This assay employs an antibody specific for rat Prolactin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Prolactin R antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Note: very low signal were obtained in normal serum and plasma

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • 组织特异性Expressed in breast, placenta, kidney, liver and pancreas.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Secreted and Membrane.
  • Information by UniProt
  • 别名
    • PRL-R
    • PRLR
    • PRLR_HUMAN
    • Prolactin receptor
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat Prolactin R ELISA Kit (PRLR) 图像

  • Representative standard curve using ab113351

实验方案

Rat Prolactin R ELISA Kit (PRLR) (ab113351)参考文献

This product has been referenced in:
  • Adedara IA  et al. Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Arch Environ Contam Toxicol N/A:N/A (2014). Read more (PubMed: 25179371) »

See 1 Publication for this product

Product Wall

Thank you for your interest in our Prolactin R Rat ELISA, ab113351. The laboratory that developed the ELISA has not tested it on milk or pituitary samples, but does not expect that there will be any problems. We recommend following the kit protocol sug...

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The laboratory has confirmed that this kit will not detect mouse Prolactin R. The rabbit polyclonal capture antibody will, but not the mouse monoclonal detection antibody.

Gracias por contactarnos.


El anticuerpo usado como captura cubriendo la placa procede de cabra, y es policlonal. El otro anticuerpo, el de detección se ha producido en ratón, y es monoclonal.

Espero haberte ayudad...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"