大鼠PAI1 ELISA试剂盒(SERPINE1) (ab198509)
Key features and details
- Sensitivity: 0.079 ng/ml
- Range: 0.1 ng/ml - 50 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Rat
概述
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产品名称
大鼠PAI1 ELISA试剂盒(SERPINE1)
参阅全部 PAI1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Known Conc 20 0.957ng/ml 0.088 9.24% Known Conc 20 2.25ng/ml 0.134 5.97% Known Conc 20 15.6ng/ml 1.01 6.48% 批次间 样品 n Mean SD CV% Known Conc 10 0.926ng/ml 0.113 12.2% Known Conc 10 1.4ng/ml 0.096 6.86% Known Conc 10 9.17ng/ml 0.858 9.36% -
样品类型
Serum, Plasma -
检测类型
Quantitative -
灵敏度
0.079 ng/ml -
范围
0.1 ng/ml - 50 ng/ml -
回收率
104 %
特定样本回收率 样品类型 平均% 范围 Spike 104 97% - 108% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat
不与反应: Sheep -
产品概述
Abcam’s PAI1 (SERPINE1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active PAI1 (SERPINE1) in rat plasma and serum.
Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate and will not be detected. Unbound PAI1 samples are washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI1 present in the samples, is then bound by the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-rabbit horseradish peroxidase secondary antibody 1 vial Anti-rat PAI-1 primary antibody 1 vial Rat PAI-1 activity standard 1 vial TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests -
研究领域
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功能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
组织特异性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
疾病相关
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
细胞定位
Secreted. - Information by UniProt
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别名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
数据库链接
- Entrez Gene: 24617 Rat
- SwissProt: P20961 Rat
- Unigene: 29367 Rat
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab198509 被引用在 3 文献中.
- Wang C et al. Vepoloxamer Enhances Fibrinolysis of tPA (Tissue-Type Plasminogen Activator) on Acute Ischemic Stroke. Stroke 50:3600-3608 (2019). PubMed: 31587657
- Grasa-López A et al. Undaria pinnatifida and Fucoxanthin Ameliorate Lipogenesis and Markers of Both Inflammation and Cardiovascular Dysfunction in an Animal Model of Diet-Induced Obesity. Mar Drugs 14:N/A (2016). Rat . PubMed: 27527189
- Jiang H et al. Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies. Sci Rep 6:30464 (2016). Rat . PubMed: 27456456