大鼠Osteopontin ELISA试剂盒(ab205076)
Key features and details
- Sensitivity: 0.02 ng/ml
- Range: 0.06 ng/ml - 4 ng/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Osteopontin ELISA试剂盒
参阅全部 Osteopontin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 10% -
样品类型
Urine, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.02 ng/ml -
范围
0.06 ng/ml - 4 ng/ml -
回收率
> 85 %
特定样本回收率 样品类型 平均% 范围 Serum > 85 0.06ng/ml - 4ng/ml -
检测时间
3h 20m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Osteopontin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Osteopontin protein in rat plasma, serum and urine.
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说明
In this assay the Osteopontin present in samples reacts with the anti-Osteopontin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Osteopontin, is added and complexes are formed. Following a wash step, horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Osteopontin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Osteopontin in the test sample. The quantity of Osteopontin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X Detection Antibody Conjugate 1 x 150µl 100X HRP-Streptavidin 1 x 150µl 1X Diluent Concentrate 1 x 60ml 20X Wash Buffer Concentrate 1 x 50ml Anti-Rat Osteopontin ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Rat Osteopotin Calibrator (Lyophilized) 1 vial Stop Solution 1 x 12ml -
研究领域
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功能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
组织特异性
Bone. Found in plasma. -
序列相似性
Belongs to the osteopontin family. -
翻译后修饰
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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别名
- BNSP
- Bone sialoprotein 1
- Bone sialoprotein I
see all -
数据库链接
- Entrez Gene: 25353 Rat
- SwissProt: P08721 Rat
- Unigene: 8871 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab205076 被引用在 1 文献中.
- Jiang P et al. Tiron ameliorates high glucose-induced cardiac myocyte apoptosis by PKCδ-dependent inhibition of osteopontin. Clin Exp Pharmacol Physiol 44:760-770 (2017). PubMed: 28394420