特异性ab99604 reacts with Mouse IgG1 as demonstrated by ELISA. Minimal reaction to Human, Goat and Rabbit IgG. Each batch is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000.
Use at an assay dependent dilution.
Rat Anti-Mouse IgG1 (Biotin), pre-adsorbed 图像
Flow Cytometry - Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (Biotin), pre-adsorbed (ab99604)
Top: (Negative control) ab99604 staining for Mouse IgG1 in Human blood at 1µg/106 cells.
Bottom: ab99604 staining in Human blood incubated with mouse anti-Human CD3 (IgG1 isotype) and Streptavidin-FITC.
Rat Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)参考文献
has not yet been referenced specifically in any publications.