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Our Abpromise guarantee covers the use of ab99632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|ELISA||1/1000 - 1/2000.|
|WB||Use at an assay dependent concentration.|
Comparison of an anti-mouse secondary antibody recognizing the H&L chain versus (ab97023) versus a mouse light chain specific secondary antibody (ab99632)
Left blot: AbExcel Goat polyclonal Secondary Antibody to Mouse IgG – H&L (HRP) (ab97023), 1/50,000
Lane 1: 0.5 µg mouse monoclonal was loaded
Lane 2: 0.25 µg mouse monoclonal was loaded
Lane 3: 0.125 µg mouse monoclonal was loaded
Right blot: Rat monoclonal Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632),1/10,000
Lane 1: 2 µg mouse monoclonal was loaded
Lane 2: 1 µg mouse monoclonal was loaded
Lane 3: 0.1 µg mouse monoclonal was loaded