概述

  • 产品名称大鼠MMP8 ELISA试剂盒
    参阅全部 MMP8 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    0.05 ng/ml - 40 ng/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.42 83% - 108%
    Serum 109.78 99% - 118%
    Plasma 107.6 98% - 118%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Can degrade fibrillar type I, II, and III collagens.
  • 组织特异性Neutrophils.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • 别名
    • CLG 1
    • CLG1
    • Collagenase 1
    • Collagenase 1 neutrophil
    • HNC
    • Matrix metallopeptidase 8 (neutrophil collagenase)
    • Matrix metalloprotease 8
    • Matrix metalloproteinase-8
    • MMP 8
    • MMP-8
    • Mmp8
    • MMP8_HUMAN
    • Neutrophil collagenase
    • PMNL CL
    • PMNL collagenase
    • PMNL-CL
    • PMNLCL
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat MMP8 ELISA Kit 图像

  • Representative standard curve using ab100779

  • Representative standard curve using ab100779

实验方案

Rat MMP8 ELISA Kit (ab100779)参考文献

This product has been referenced in:
  • Monaghan M  et al. A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling. Mol Ther 22:786-96 (2014). Read more (PubMed: 24402185) »

See 1 Publication for this product

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Thank you for your patience.

The lab let me know the following:

ab100609 : Cross reactivity with mouse is unknown. If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal.

ab100779 : Untes...

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Thank you for contacting us.

I have contacted the lab regarding the cross-reactivity of the 2 kits and will let you know what I find out.

As for the testing discount program, we have the following general information:

For...

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Thank you for your enquiry.

ab100779 does not measure MMP8 activity. It is a sandwich ELISA assay that just quantitates amount of protein present.

I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"