大鼠MCP1 ELISA试剂盒(CCL2) (ab100777)

概述

  • 产品名称大鼠MCP1 ELISA试剂盒(CCL2)
    参阅全部 MCP1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    24.67 pg/ml - 18000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101.4 86% - 105%
    Serum 95.39 84% - 103%
    Plasma 92.44 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C-C motif chemokine 2
    • CCL2
    • CCL2_HUMAN
    • Chemokine (C C motif) ligand 2
    • GDCF 2
    • GDCF-2
    • GDCF2
    • HC11
    • HSMCR30
    • JE
    • MCAF
    • MCP 1
    • MCP-1
    • MCP1
    • MGC9434
    • Monocyte chemoattractant protein 1
    • Monocyte chemotactic and activating factor
    • Monocyte chemotactic protein 1
    • Monocyte secretory protein JE
    • SCYA2
    • Small inducible cytokine A2
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je)
    • Small inducible cytokine subfamily A (Cys Cys), member 2
    • Small-inducible cytokine A2
    • SMC CF
    • SMC-CF
    • SMCCF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat MCP1 ELISA Kit (CCL2) 图像

  • Representative standard curve using ab100777

  • Representative standard curve using ab100777

实验方案

Rat MCP1 ELISA Kit (CCL2) (ab100777)参考文献

ab100777 has not yet been referenced specifically in any publications.

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I have addressed your questions below.
1. Firstly I need to use the kit to detect urinary MCP-1, so which Diluent should I use: Diluent A or B?

Assay Diluent A is optimized specifically for only se...

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Although we have not tested and validated this ELISA kit with urine samples, based on previous testing with our ELISA kits the Rat MCP1 ELISA is very likely to work for urine.

Some general guidelines on ...

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Thank you for your enquiry. I apologize for the delay in replying.

The two kits use the same antibodies but a few other reagents in each kit differ, to optimize the assay for different sample types. Product ab100777 is best for plasma, serum...

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