大鼠MCP1 ELISA试剂盒(CCL2) (ab100777)

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概述

  • 产品名称
    大鼠MCP1 ELISA试剂盒(CCL2)
    参阅全部 MCP1 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    24.67 pg/ml - 18000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101.4 86% - 105%
    Serum 95.39 84% - 103%
    Plasma 92.44 83% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 序列相似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • C-C motif chemokine 2
    • CCL2
    • CCL2_HUMAN
    • Chemokine (C C motif) ligand 2
    • GDCF 2
    • GDCF-2
    • GDCF2
    • HC11
    • HSMCR30
    • JE
    • MCAF
    • MCP 1
    • MCP-1
    • MCP1
    • MGC9434
    • Monocyte chemoattractant protein 1
    • Monocyte chemotactic and activating factor
    • Monocyte chemotactic protein 1
    • Monocyte secretory protein JE
    • SCYA2
    • Small inducible cytokine A2
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je)
    • Small inducible cytokine subfamily A (Cys Cys), member 2
    • Small-inducible cytokine A2
    • SMC CF
    • SMC-CF
    • SMCCF
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab100777 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    Rat MCP1 ELISA Kit (CCL2) 图像

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab100777

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab100777

    实验方案

    Rat MCP1 ELISA Kit (CCL2) (ab100777)参考文献

    ab100777 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab100777？请让我们知道，以便我们可以引用本数据表中的参考文章。

    Product Wall

    提交 ab100777 的 abreview 提交 ab100777 的问题

    6 Abreviews or Q&A

    10
    Votes: Highest First

    I'm glad to hear from you. I prefer to send a new kit since we have not been able to analyze the samples for the lack of reagent in the previous kit. We are very pleased with the way to resolve this incident.
    Best regards,

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product was not supplied in full and for the inconvenience this has caused. As re...

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    We acquired the MCP1 rat ELISA kit 1*96 well plate, reference ab100777, lot: GR85755-5. The item G (Streptavidin-HRP) of this kit should be contain 200 uL and only contained 10 uL.

    Thank you for taking time to contact us.

    I am sorry to hear that this kit was not supplied to you in full.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compe...

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    shortage of a component

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I h...

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    Dear staff, I have already purchased and received the ab100777 MCP-1 ELISA kit and was wondering if you could clarify some of the instructions. 1. Firstly I need to use the kit to detect urinary MCP-1, so which Diluent should I use: Diluent A or B? 2. ...

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    Thank you for your enquiry.

    I have addressed your questions below.
    1. Firstly I need to use the kit to detect urinary MCP-1, so which Diluent should I use: Diluent A or B?

    Assay Diluent A is optimized specifically for only se...

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    Dear staff
    I am interested in purchasing product ab100777 (the Rat MCP-1 immunoassay) and was wondering whether this kit is suitable for detecting MCP-1 in rat urine?
    Many thanks

    Thank you for contacting us.

    Although we have not tested and validated this ELISA kit with urine samples, based on previous testing with our ELISA kits the Rat MCP1 ELISA is very likely to work for urine.

    Some general guidelines on ...

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    I would like to know the differences between
    MCP1 Rat ELISA Kit (a b100777) plasma
    MCP1 Rat ELISA Kit (a b100778) extract
    Why can I not use the same for both samples?

    Thank you for your enquiry. I apologize for the delay in replying.

    The two kits use the same antibodies but a few other reagents in each kit differ, to optimize the assay for different sample types. Product ab100777 is best for plasma, serum...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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