大鼠Liver线粒体裂解液(ab110346)

概述

  • 产品名称大鼠Liver线粒体裂解液
    参阅全部 Rat Liver 细胞裂解液
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.


    2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • 经测试应用适用于: SDS-PAGE, WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • 裂解物说明2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

    应用

    Our Abpromise guarantee covers the use of ab110346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    SDS-PAGE Use at an assay dependent dilution.
    WB Use at an assay dependent dilution.

    Rat Liver Mitochondrial lysate (ab110346)参考文献

    ab110346 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. Please find below the further requested information.

    The composition is 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose, pH 7.8

    The concentration of mitochondrial lysate is 2mg at 5mg/ml.

    I hop...

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    Thank you for your enquiry. As far as we know the expression of Thioredoxin 2 in rat liver mitochondria has not been determined. However, there is a western blot on the datasheet for ab16856 illustrating that 293T cell lysates express Thioredoxi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"