大鼠Leptin ELISA试剂盒(ab100773)

概述

  • 产品名称大鼠Leptin ELISA试剂盒
    参阅全部 Leptin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    10.97 pg/ml - 8000 pg/ml
  • 复检

    84 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 75.43 68% - 89%
    Serum 92.32 83% - 102%
    Plasma 84.25 75% - 94%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 疾病相关Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性Belongs to the leptin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • FLJ94114
    • LEP
    • LEP_HUMAN
    • LEPD
    • Leptin
    • Leptin (murine obesity homolog)
    • Leptin (obesity homolog, mouse)
    • Leptin Murine Obesity Homolog
    • Leptin Precursor Obesity Factor
    • OB
    • Obese protein
    • Obese, mouse, homolog of
    • Obesity
    • Obesity factor
    • Obesity homolog mouse
    • Obesity Murine Homolog Leptin
    • OBS
    • OTTHUMP00000212285
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat Leptin ELISA Kit 图像

  • Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample

  • Rat Leptin measured in biofluids showing quantity (ng) per mL of tested sample

  • Standard curve: mean of duplicates (+/-SD) with background readings subtracted

实验方案

Rat Leptin ELISA Kit (ab100773)参考文献

ab100773 has not yet been referenced specifically in any publications.

Product Wall

Abreview for Leptin Rat ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Rat Serum (Blood)
Specification: Blood
Amount of Sample: 50 µg
Type of Kit Used: ELISA
Positive control: rat serum (diabetic)
Did you use the Abcam Kit protocol?: Yes
Username

Dr. Asmat Salim

Verified customer

提交于 May 07 2012

Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"