大鼠KIM-1 TIM-1 ELISA试剂盒(ab119597)

概述

  • 产品名称大鼠KIM-1 TIM-1 ELISA试剂盒
    参阅全部 TIM 1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Tissue Homogenate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    31.2 pg/ml - 2000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s rat KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat KIM-1 (TIM-1) in cell culture supernatants, tissue homogenates, serum, plasma (heparin, EDTA) and urine.

    A KIM-1 (TIM-1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat KIM-1 (TIM-1) amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • 组织特异性Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • 序列相似性Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD365
    • HAVCR
    • HAVCR 1
    • HAVcr-1
    • Havcr1
    • Hepatitis A virus cellular receptor 1
    • Kidney injury molecule 1
    • KIM 1
    • KIM-1
    • T cell immunoglobin domain and mucin domain protein 1
    • T cell immunoglobulin mucin family member 1
    • T cell immunoglobulin mucin receptor 1
    • T-cell immunoglobulin and mucin domain-containing protein 1
    • T-cell membrane protein 1
    • TIM
    • TIM-1
    • TIM1
    • TIMD 1
    • TIMD-1
    • TIMD1
    • TIMD1_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat KIM-1 TIM-1 ELISA Kit 图像

  • Representaive Standard Curve using ab119597

实验方案

Rat KIM-1 TIM-1 ELISA Kit (ab119597)参考文献

ab119597 has not yet been referenced specifically in any publications.

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To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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I can confirm that PBS and homogenization will be absolutely fine and can be used flawlessly with the kit. Using tissue lysis buffer/solution is even better but PBS alone should work perfectly well.

The pH of rat urine is close to 7.0, and there is no need to adjust the pH before use with the kit.

Thank you for contacting us.
We have several TIM1 antibodies (TIM1 is a synonym for KIM1).
Two of these antibodies were tested in IHC with paraffin sections with human and mouse, but not rat.
If you like to try using these antibodies in ...

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I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Li...

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Thank you for contacting us. It is my pleasure to help.

We do offer a KIM1 rat ELISA kit as well. This kit has a similar system containing one plate made of twelve 8 well strips. The product number is ab119597 and may be found at the follo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"