大鼠IL-4 ELISA试剂盒(Interleukin-4) (ab46073)

概述

  • 产品名称大鼠IL-4 ELISA试剂盒(Interleukin-4)
    参阅全部 IL4 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 18 379.3pg/ml 13.5 2.5%
    B 19 532.5pg/ml 5.99 1.6%
    批次间
    样品 n Mean SD CV%
    A 6 128.6pg/ml 7.6 5.9%
    B 6 532.2pg/ml 20.6 3.8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    4h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-4 (Interleukin-4) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in cell culture supernatants, buffered solutions and plasma.

    A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentRations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substRate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentRation of IL-4 present in the samples.

    This kit will recognize both endogenous and recombinant rat IL-4.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • 细胞定位Secreted
  • 别名
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-4 ELISA Kit (Interleukin-4) 图像

  •  

    Representative Standard Curve using ab46073

实验方案

Rat IL-4 ELISA Kit (Interleukin-4) (ab46073)参考文献

ab46073 has not yet been referenced specifically in any publications.

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ab46073:
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