大鼠IL-4 ELISA试剂盒(Interleukin-4) (ab100770)

概述

  • 产品名称大鼠IL-4 ELISA试剂盒(Interleukin-4)
    参阅全部 IL4 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    0.66 pg/ml - 160 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.69 85% - 104%
    Serum 96.79 84% - 103%
    Plasma 94.86 82% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-4 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • 细胞定位Secreted
  • 别名
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-4 ELISA Kit (Interleukin-4) 图像

  • Representative standard curve using ab100770

  • Representative standard curve using ab100770

实验方案

Rat IL-4 ELISA Kit (Interleukin-4) (ab100770)参考文献

ab100770 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

Yes, Assay Diluent A contains sodium azide which inhibits HRP activity. Therefore in this case 1X Assay Diluent B should always be used to prepare the HRP-Streptavidin and detection antibody regardless of the sam...

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The lab adviced the following:

While serum and plasma samples maybe run undiluted, we do not usually recommend doing so. The reason is not because Assay Diluent A needs to be present in every sample but that testing undiluted serum/plasma...

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I have the following information for you regarding the kits we discussed:


ab46073:
We have no data regarding normal values observed with IL-6 and IL-4 in rat but as free circulating cytokines are rather dangerous for living ani...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"