96
%
样品类型 | 平均% | 范围 |
---|---|---|
Cell culture supernatant | 97.69 | 85% - 104% |
Serum | 96.79 | 84% - 103% |
Plasma | 94.86 | 82% - 102% |
Abcam’s IL-4 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for rat IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
组件 | 1 x 96 tests |
---|---|
20X Wash Buffer Concentrate | 1 x 25ml |
5X Assay Diluent B | 1 x 15ml |
600X HRP-Streptavidin Concentrate | 1 x 200µl |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-rat IL-4 (lyophilized) | 2 vials |
IL-4 Microplate (12 x 8 wells) | 1 unit |
Recombinant rat IL-4 Standard | 2 vials |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
ab100770 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"