大鼠IL-4 ELISA试剂盒(Interleukin-4) (ab100770)

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概述

  • 产品名称
    大鼠IL-4 ELISA试剂盒(Interleukin-4)
    参阅全部 IL4 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    0.66 pg/ml - 160 pg/ml
  • 回收率

    96 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 97.69 85% - 104%
    Serum 96.79 84% - 103%
    Plasma 94.86 82% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-4 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat IL-4 (lyophilized) 2 vials
    IL-4 Microplate (12 x 8 wells) 1 unit
    Recombinant rat IL-4 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • 细胞定位
    Secreted
  • 别名
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab100770 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    图片

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100770

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100770

    实验方案

    文献

    ab100770 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab100770？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    3 reviews or Q&A

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    regarding IL-4 rat elisa (ab100770) -

    In the protocol (page 7) #6. PREPARATION OF REAGENTS, step 6. says to add diluent B to prepare the detection antibody and step 7 says to add diluent B to dilute HRP. My question is this, should you use d...

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    Thank you for contacting us.

    Yes, Assay Diluent A contains sodium azide which inhibits HRP activity. Therefore in this case 1X Assay Diluent B should always be used to prepare the HRP-Streptavidin and detection antibody regardless of the sam...

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    Can serum samples be run "neat" (without dilution) in the IL-4 Elisa - ab100770?


    The lab adviced the following:

    While serum and plasma samples maybe run undiluted, we do not usually recommend doing so. The reason is not because Assay Diluent A needs to be present in every sample but that testing undiluted serum/plasma...

    Read More

    What is the normal amount of rat IL4 in serum?
    What is the normal amount of rat IL6 in serum?
    What is the recommended dilution range?


    I have the following information for you regarding the kits we discussed:


    ab46073:
    We have no data regarding normal values observed with IL-6 and IL-4 in rat but as free circulating cytokines are rather dangerous for living ani...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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