大鼠IL-2 ELISA试剂盒(Interleukin-2) (ab100769)

概述

  • 产品名称大鼠IL-2 ELISA试剂盒(Interleukin-2)
    参阅全部 IL2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.1 ng/ml
  • 范围
    0.12 ng/ml - 30 ng/ml
  • 复检

    85 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 78.52 67% - 89%
    Serum 98.73 88% - 109%
    Plasma 80.35 70% - 91%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-2 (Interleukin-2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in serum, plasma and cell culture supernatants. (Rat IL-2 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 疾病相关Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 序列相似性Belongs to the IL-2 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-2 ELISA Kit (Interleukin-2) 图像

  • Representative standard curve using ab100769

  • Representative standard curve using ab100769

实验方案

Rat IL-2 ELISA Kit (Interleukin-2) (ab100769)参考文献

ab100769 has not yet been referenced specifically in any publications.

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