大鼠IL-2 ELISA试剂盒(Interleukin-2) (ab100769)

概述

  • 产品名称
    大鼠IL-2 ELISA试剂盒(Interleukin-2)
    参阅全部 IL2 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 0.1 ng/ml
  • 范围
    0.12 ng/ml - 30 ng/ml
  • 复检

    85 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 78.52 67% - 89%
    Plasma 80.35 70% - 91%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-2 (Interleukin-2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2 in plasma and cell culture supernatants. (Rat IL-2 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat IL-2 in normal serum with ab100769, only in serum spiked with Rat IL-2.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100769

  • Representative standard curve using ab100769

实验方案

文献

ab100769 has not yet been referenced specifically in any publications.

客户评价及客户问答

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I have contacted the lab and received the following information:

While ab100769was neither designed nor validated with any lysate samples, the kit is very accommodating to many different samples types s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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