大鼠IL-17A ELISA试剂盒(Interleukin-17) (ab119536)

概述

  • 产品名称
    大鼠IL-17A ELISA试剂盒(Interleukin-17)
    参阅全部 IL17A 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 7 8.5%
    批次间
    样品 n Mean SD CV%
    Overall 7 7.6%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    1 pg/ml
  • 范围
    1.6 pg/ml - 100 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 92% - 112%
    Serum 34% - 44%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-17A (Interleukin-17) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed


    for accurate quantitative measurement of Rat IL-17 (Interleukin-17)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin).


    IL-17 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-17 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17 captured on the plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-rat IL17 monoclonal antibody 1 x 70µl
    Microplate coated with monoclonal antibody to rat IL-17 (12 x 8 wells) 1 unit
    Rat IL17 Standard Lyophilized (200 pg/mL on reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 组织特异性
    Restricted to activated memory T-cells.
  • 序列相似性
    Belongs to the IL-17 family.
  • 翻译后修饰
    Found both in glycosylated and nonglycosylated forms.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • OTTHUMP00000016597
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119536.

实验方案

文献

ab119536 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your patience in this matter. There will be a big difference in the height of the OD values measured before and after addition of the stop solution, so you should take your readings AFTER the stop solution.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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