大鼠IL-17 ELISA试剂盒(Interleukin-17) (ab119536)

概述

  • 产品名称大鼠IL-17 ELISA试剂盒(Interleukin-17)
    参阅全部 IL17 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 7 8.5%
    批次间
    样品 n Mean SD CV%
    Overall 7 7.6%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1 pg/ml
  • 范围
    1.6 pg/ml - 100 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 92% - 112%
    Serum 34% - 44%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-17 (Interleukin-17) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed

    for accurate quantitative measurement of Rat IL-17 (Interleukin-17)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    IL-17 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-17 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17 captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 组织特异性Restricted to activated memory T-cells.
  • 序列相似性Belongs to the IL-17 family.
  • 翻译后修饰Found both in glycosylated and nonglycosylated forms.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-17 ELISA Kit (Interleukin-17) 图像

  • Representative Standard Curve using ab119536.

实验方案

Rat IL-17 ELISA Kit (Interleukin-17) (ab119536)参考文献

ab119536 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in this matter. There will be a big difference in the height of the OD values measured before and after addition of the stop solution, so you should take your readings AFTER the stop solution.

I would also recommen...

Read More


Samples can vary from mouse to mouse because all mice (even within the same stains) to do not always express proteins at the same levels. Therefore a titration may be necessary.

However, if instructions in our protocol have been followed,...

Read More

Thank you for your reply.

The Anti-PPAR gamma antibody (ab27649) is provided already reconstituted in liquid form in a PBS buffer (pH 7.4) with 1% BSA and 0.02% sodium azide supplemented. The customer therefore does not need to reconstitute i...

Read More

Muchas gracias por contactarnos. Agradezco el interés en nuestros productos.

Lamentablemente, ya que tenemos en el catálogo más de 80.000 productos, nos resulta imposible guardar muestras de prueba de todos ellos para enviar como alícuotas d...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"