大鼠IL-13 ELISA试剂盒(Interleukin-13) (ab100766)

概述

  • 产品名称
    大鼠IL-13 ELISA试剂盒(Interleukin-13)
    参阅全部 IL13 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    27.43 pg/ml - 20000 pg/ml
  • 回收率

    114 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture extracts 120.4 80% - 139%
    Tissue Extracts 107.8 81% - 135%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.

    This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100766

实验方案

文献

This product has been referenced in:
  • Seiffert J  et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . Read more (PubMed: 25747867) »

See 1 Publication for this product

客户评价及客户问答

ab100766 tested now on rat serum

Good Good 4/5 (Ease of Use)
Abreviews
the product worked perfectly on rat serum with the same protocol suggested in the kit for cell culture exctracts, tissue exctracts.
Username

Vasiliki Koumaki

Verified customer

提交于 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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