大鼠IL-13 ELISA试剂盒(Interleukin-13) (ab100766)

概述

  • 产品名称大鼠IL-13 ELISA试剂盒(Interleukin-13)
    参阅全部 IL13 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    27.43 pg/ml - 20000 pg/ml
  • 复检

    114 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 120.4 80% - 139%
    Tissue Extracts 107.8 81% - 135%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.

    This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 疾病相关Allergic rhinitis
  • 序列相似性Belongs to the IL-4/IL-13 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-13 ELISA Kit (Interleukin-13) 图像

  • Representative standard curve using ab100766

实验方案

Rat IL-13 ELISA Kit (Interleukin-13) (ab100766)参考文献

ab100766 has not yet been referenced specifically in any publications.

Product Wall

ab100766 tested now on rat serum

Good Good 4/5 (Ease of Use)
Abreviews
the product worked perfectly on rat serum with the same protocol suggested in the kit for cell culture exctracts, tissue exctracts.
Username

Vasiliki Koumaki

Verified customer

提交于 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"