大鼠IL-10 ELISA试剂盒(Interleukin-10) (ab100765)

概述

  • 产品名称
    大鼠IL-10 ELISA试剂盒(Interleukin-10)
    参阅全部 IL10 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 回收率

    140 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture extracts 142.1 133% - 149%
    Tissue Extracts 138.7 127% - 146%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in cell lysates and tissue lysates.

    This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100765.

实验方案

文献

This product has been referenced in:
  • Dolatabadi HRD  et al. The Effects of Pentoxifylline on Serum Levels of Interleukin 10 and Interferon Gamma and Memory Function in Lipopolysaccharide-induced Inflammation in Rats. Adv Biomed Res 6:110 (2017). Read more (PubMed: 28904938) »
  • Payghani C  et al. The Effect of Levothyroxine on Serum Levels of Interleukin 10 and Interferon-gamma in Rat Model of Multiple Sclerosis. Adv Biomed Res 6:118 (2017). Read more (PubMed: 28989911) »

See all 4 Publications for this product

客户评价及客户问答

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I am sorry that the standard curve was non-linear in the kit you received. Can you please confirm some additional details?
1) Was the vial of Item C spun prior to opening? Did you mix thoroughly to resuspend the s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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