大鼠IL-1 beta ELISA试剂盒(Interleukin-1 beta) (ab100768)

概述

  • 产品名称大鼠IL-1 beta ELISA试剂盒(Interleukin-1 beta)
    参阅全部 IL1 beta 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 80 pg/ml
  • 范围
    68.59 pg/ml - 50000 pg/ml
  • 复检

    116 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 96.22 88% - 105%
    Tissue Extracts 136.5 126% - 145%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-1 beta (Interleukin-1 beta) Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates.

    This assay employs an antibody specific for rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性Belongs to the IL-1 family.
  • 结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 别名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-1 beta ELISA Kit (Interleukin-1 beta) 图像

  • Standard curve of Rt IL1b, with background signal subtracted (duplicates; +/- SD).
  • Rat IL-1b measured in biological fluids or in spleen lysate; background signal subtracted (duplicates +/- SD).
  • Rat IL-1b detected in cell lysates from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma). Results shown for IL-1? levels in 10e6 cells, after background signal was subtracted (duplicates +/- SD).
  • Representative standard curve using ab100768

实验方案

Rat IL-1 beta ELISA Kit (Interleukin-1 beta) (ab100768)参考文献

This product has been referenced in:
  • Kang SJ  et al. Inhibitory effects of Persicariae Rhizoma aqueous extracts on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats. Exp Ther Med 12:1563-1571 (2016). Rat . Read more (PubMed: 27588077) »
  • Mahmoud-Awny M  et al. Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-?, NF-?B and Nrf2/HO-1 Signaling Pathways. PLoS One 10:e0132497 (2015). Read more (PubMed: 26196679) »

See all 5 Publications for this product

Product Wall



I can suggest the following general tissue lysate preparation procedure to prepare samples for both the kits.




Tissue lyste samples can be prepared using most conventional methods, for example with RIPA or other formula...

Read More



Da die Tiere ja nur "durchgespült", aber nicht fixiert wurden, sollte dieses Kit mit den Leberlysaten funktionieren.

Vielen Dank für Ihre Anfrage.

Womit perfusieren Sie den die Tiere, PFA oder ein anderes Fixierungsmittel oder einfach nur PBS?

Ich freue mich auf Ihre Antwort.

Thank you for your enquiry. I can confirm that ab100767 IL1 beta Rat ELISA Kit would be the suitable kit to use with plasma samples. ab100768 IL1 beta Rat ELISA Kit is not designed for use with plasma samples. I hope this will be helpful to yo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"