99
%
样品类型 | 平均% | 范围 |
---|---|---|
Cell culture supernatant | 92.89 | 81% - 109% |
Serum | 107.2 | 95% - 115% |
Plasma | 97.55 | 89% - 108% |
Abcam’s IL-1 beta (Interleukin-1 beta) Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
组件 | 1 x 96 tests |
---|---|
200X HRP-Streptavidin Concentrate | 1 x 200µl |
20X Wash Buffer Concentrate | 1 x 25ml |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-rat IL-1 beta | 2 vials |
IL-1 beta Microplate (12 x 8 wells) | 1 unit |
Recombinant rat IL-1 beta Standard | 2 vials |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"