大鼠IL-1 beta ELISA试剂盒(Interleukin-1 beta) (ab100767)
- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称大鼠IL-1 beta ELISA试剂盒(Interleukin-1 beta)
参阅全部 IL1 beta 试剂盒 -
检测方法Colorimetric
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样品类型Cell culture supernatant, Serum, Plasma
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检测类型Sandwich (quantitative)
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灵敏度< 80 pg/ml
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范围68.59 pg/ml - 50000 pg/ml
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回收率
99 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 92.89 81% - 109% Serum 107.2 95% - 115% Plasma 97.55 89% - 108% -
实验步骤Multiple steps standard assay
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种属反应性与反应: Rat
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产品概述
Abcam’s IL-1 beta (Interleukin-1 beta) Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
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经测试应用适用于: Sandwich ELISAmore details
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平台Microplate
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-rat IL-1 beta 2 vials IL-1 beta Microplate (12 x 8 wells) 1 unit Recombinant rat IL-1 beta Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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序列相似性Belongs to the IL-1 family.
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结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
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细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
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别名
- Catabolin
- H1
- Hematopoietin 1
see all -
数据库链接
- SwissProt: Q63264 Rat
- Unigene: 9869 Rat
应用
Our Abpromise guarantee covers the use of ab100767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
图片
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Typical dataRepresentative standard curve using ab100767
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typical dataRepresentative standard curve using ab100767
实验方案
文献
This product has been referenced in:
- Ho D et al. Maternal-placental-fetal biodistribution of multimodal polymeric nanoparticles in a pregnant rat model in mid and late gestation. Sci Rep 7:2866 (2017). Read more (PubMed: 28588270) »
- Jiang YH et al. Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation. PLoS One 10:e0133971 (2015). Read more (PubMed: 26230083) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"