大鼠IL-1 alpha ELISA试剂盒(Interleukin-1 alpha) (ab113350)

概述

  • 产品名称
    大鼠IL-1 alpha ELISA试剂盒(Interleukin-1 alpha)
    参阅全部 IL1 alpha 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    11 pg/ml - 8000 pg/ml
  • 复检

    81 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 87.48 76% - 95%
    Serum 79.95 69% - 88%
    Plasma 75.73 68% - 84%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IL-1 alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 alpha in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab113350

实验方案

文献

This product has been referenced in:
  • Zhang X  et al. Risk of radiation-induced pneumonitis after helical and static-port tomotherapy in lung cancer patients and experimental rats. Radiat Oncol 10:195 (2015). Read more (PubMed: 26382926) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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