大鼠IgG2a, kappa单克隆抗体[RTK2758] (Low endotoxin, Azide Free) (ab18450)

概述

  • 产品名称大鼠IgG2a, kappa单克隆抗体[RTK2758] (Low endotoxin, Azide Free)
    参阅全部 Rat 亚类对照
  • 共轭说明Please note that this antibody is unconjugated.
  • 经测试应用适用于: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
  • 免疫原

    Trinitrophenol + KLH

  • 常规说明


    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
    0.2 µm filter sterilized. Endotoxin level is <0.01 ng/µg protein
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab18450 is LEAF™ Purified. This a low endotoxin – sodium azide free formulation. ab18450 was 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein. LEAF™ Purified formulation is typically used for bioassays, capture antibody for ELISPOT application, and in vivo studies such as Blocking/Neutralization.
  • Isotype control说明ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • 克隆单克隆
  • 克隆编号RTK2758
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域
  • 别名
    • Rat Isotype Control

应用

Our Abpromise guarantee covers the use of ab18450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab18450is a negative isotype control



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) (ab18450)参考文献

This product has been referenced in:
  • Daschkey S  et al. MicroRNAs distinguish cytogenetic subgroups in pediatric AML and contribute to complex regulatory networks in AML-relevant pathways. PLoS One 8:e56334 (2013). IP . Read more (PubMed: 23418555) »
  • Hausmann M  et al. BCL-2 Modifying Factor (BMF) Is a Central Regulator of Anoikis in Human Intestinal Epithelial Cells. J Biol Chem 286:26533-40 (2011). Read more (PubMed: 21673109) »

See all 3 Publications for this product

Product Wall

Expiration date: 25-04-2012
I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
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The subisotype ofab86488 is IgG2a. Thus, the recommended isotype control to be used in flow cytometry would be ab18450.

I hope this information is helpful to you. Please do not hesitate to contact us i...

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Flow cytometry has not yet been tested with this antibody. Therefore, we do not have data to show if and how well it would work in this application. But for UNTESTED species and/or applications, we have establish...

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Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice p...

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Thank you for your enquiry. To our knowledge this antibody has yet to be tested in-vivo. If you decide to purchase this antibody for testing in applications for which we don't have any information, please let us know how you get on. In return we will a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"