概述

  • 产品名称大鼠IFN gamma ELISA试剂盒
    参阅全部 Interferon gamma 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 19 365.1pg/ml 12.7 2.9%
    B 20 554.6pg/ml 16.4 3.4%
    批次间
    样品 n Mean SD CV%
    A 6 148.2pg/ml 6.6 4.5%
    B 6 560.7pg/ml 29.5 5.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IFN gamma Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in cell culture supernatants, buffered solutions and plasma.

    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

    This kit will recognize both endogenous and recombinant rat IFN gamma

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • 别名
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat IFN gamma ELISA Kit 图像

  • Representative Standard Curve using ab46107

实验方案

Rat IFN gamma ELISA Kit (ab46107)参考文献

ab46107 has not yet been referenced specifically in any publications.

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