概述

  • 产品名称
    大鼠IFN gamma ELISA试剂盒
    参阅全部 Interferon gamma 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 100 pg/ml
  • 范围
    123 pg/ml - 30000 pg/ml
  • 回收率

    98 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 99.29 89% - 111%
    Plasma 92.63 78% - 104%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s IFN gamma Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IFN gamma in plasma and cell culture supernatants. (Rat IFN gamma concentration in normal plasma is pretty low, it may not be detected in this assay).


    This assay employs an antibody specific for IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat IFN-gamma in normal serum with ab113349, only in serum spiked with Rat IFN-gamma.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab113349

  • Representative standard curve using ab113349

实验方案

文献

ab113349 has not yet been referenced specifically in any publications.

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