大鼠Heart线粒体裂解液(ab110347)

概述

  • 产品名称大鼠Heart线粒体裂解液
    参阅全部 Rat Heart 细胞裂解液
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.


    2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • 经测试应用适用于: SDS-PAGE, WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • 裂解物说明2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

    应用

    Our Abpromise guarantee covers the use of ab110347 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    SDS-PAGE Use at an assay dependent dilution.
    WB Use at an assay dependent dilution.

    Rat Heart Mitochondrial lysate (ab110347)参考文献

    ab110347 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"