大鼠Haptoglobulin ELISA试剂盒(ab108857)

概述

  • 产品名称
    大鼠Haptoglobulin ELISA试剂盒
    参阅全部 Haptoglobin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.5%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Competitive
  • 灵敏度
    = 0.9 µg/ml
  • 范围
    0.937 µg/ml - 60 µg/ml
  • 复检

    99 %

  • 检测时间
    3h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Haptoglobin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.

    A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of haptoglobin captured in plate.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    2X Biotinylated Haptoglobulin (Lyophilized) 1 vial
    Chromogen Substrate 1 x 8ml
    Rat Haptoglobulin Microplate (12 x 8 well strips) 1 unit
    Rat Haptoglobulin Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • 组织特异性
    Expressed by the liver and secreted in plasma.
  • 疾病相关
    Defects in HP are the cause of anhaptoglobinemia (AHP) [MIM:614081]. AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin.
  • 序列相似性
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • binding peptide
    • BP
    • haptoglobin alpha 1S beta
    • haptoglobin alpha 2FS beta
    • haptoglobin alpha polypeptide
    • Haptoglobin beta chain
    • haptoglobin beta polypeptide
    • HP
    • HP2ALPHA2
    • HPA1S
    • HPT_HUMAN
    • Zonulin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

图片

  • Representative Standard Curve using ab108857.

实验方案

文献

ab108857 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us and sorry for the delay in getting back to you. Your customer is correct in thinking that the Haptoglobin and Haptoglobulin refer to the same protein. I am sorry that our datasheets have caused confusion. I will be looki...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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