大鼠Fractalkine ELISA试剂盒(CX3CL1) (ab100760)

概述

  • 产品名称
    大鼠Fractalkine ELISA试剂盒(CX3CL1)
    参阅全部 CX3CL1 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.35 83% - 104%
    Serum 94.54 83% - 103%
    Plasma 93.46 84% - 105%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Fractalkine Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fractalkine in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Rat Fractalkine coated on a 96-well plate. Standards and samples are pipetted into the wells and Fractalkine present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fractalkine antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fractalkine bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    240X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Rat Fractalkine 2 vials
    Fractalkine Microplate (12 x 8 wells) 1 unit
    Recombinant Rat Fractalkine Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.
  • 别名
    • A 152E5.2
    • AB030188
    • ABCD 3
    • ABCD3
    • C X3 C motif chemokine 1
    • C3Xkine
    • Chemokine (C X3 C motif) ligand 1
    • Chemokine C X3 C motif ligand 1
    • Chemokine CX3C Motif Ligand 1
    • CX3C membrane anchored chemokine
    • CXC3
    • CXC3C
    • FKN
    • Fractalkine
    • Neurotactin
    • NTN
    • NTT
    • SCYD 1
    • SCYD1
    • Small inducible cytokine D1
    • Small inducible cytokine subfamily D (Cys X3 Cys), member 1
    • Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
    • Small inducible cytokine subfamily D member 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100760

  • Representative Standard Curve using ab100760

实验方案

文献

ab100760 has not yet been referenced specifically in any publications.

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