大鼠Fibrinogen ELISA试剂盒(ab108846)
Key features and details
- Sensitivity: 0.56 µg/ml
- Range: 1.563 µg/ml - 25 µg/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Rat
概述
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产品名称
大鼠Fibrinogen ELISA试剂盒
参阅全部 Fibrinogen 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.4% 批次间 样品 n Mean SD CV% Overall 10.1% -
样品类型
Plasma -
检测类型
Competitive -
灵敏度
= 0.56 µg/ml -
范围
1.563 µg/ml - 25 µg/ml -
回收率
96 %
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检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s Fibrinogen Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen in plasma.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Rat Fibrinogen (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 7ml Fibrinogen Microplate (12 x 8 well strips) 1 unit Fibrinogen Standard 2 vials Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
组织特异性
Plasma. -
疾病相关
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻译后修饰
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
细胞定位
Secreted. - Information by UniProt
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别名
- FGA
- FGB
- FGG
see all -
数据库链接
- Entrez Gene: 361969 Rat
- Entrez Gene: 24366 Rat
- Entrez Gene: 24367 Rat
- SwissProt: P14480 Rat
- SwissProt: P02680 Rat
- SwissProt: P06399 Rat
- Unigene: 98846 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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