大鼠Fas Ligand ELISA试剂盒(CD95L) (ab100759)

概述

  • 产品名称
    大鼠Fas Ligand ELISA试剂盒(CD95L)
    参阅全部 Fas Ligand 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 90 pg/ml
  • 范围
    82.3 pg/ml - 60000 pg/ml
  • 回收率

    100 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 92.64 81% - 98%
    Plasma 118.9 109% - 125%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Fas Ligand (CD95L) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fas Ligand (CD95L) in plasma and cell culture supernatants. (Rat Fas Ligand concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Rat Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Rat Fas Ligand in normal serum with ab100759, only in serum spiked with Rat Fas Ligand.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    160X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-rat Fas Ligand 2 vials
    Fas Ligand Microplate (12 x 8 wells) 1 unit
    Recombinant Rat Fas Ligand Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.
  • 细胞定位
    Cell Membrane (single-pass type II membrane protein) and Secreted
  • 别名
    • Apoptosis (APO 1) antigen ligand 1
    • Apoptosis antigen ligand
    • APT1LG1
    • APTL
    • CD178
    • CD95 L
    • CD95 ligand
    • CD95L
    • CD95L protein
    • Fas antigen ligand
    • FASL
    • FASLG
    • TNF superfamily member 6
    • TNFSF6
    • Tumor necrosis factor (ligand) superfamily member 6
    • Tumor necrosis factor ligand superfamily member 6
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100759.

  • Representative Standard Curve using ab100759.

实验方案

文献

ab100759 has not yet been referenced specifically in any publications.

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