大鼠CNTF ELISA试剂盒(ab100758)

概述

  • 产品名称
    大鼠CNTF ELISA试剂盒
    参阅全部 CNTF 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    8.23 pg/ml - 2000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.3 89% - 108%
    Serum 105.6 93% - 112%
    Plasma 104.2 92% - 110%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s CNTF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CNTF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat CNTF 2 vials
    CNTF Microplate (12 strips x 8 wells) 1 unit
    Recombinant rat CNTF Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
  • 细胞定位
    Cytoplasmic
  • 别名
    • AI429687
    • Ciliary Neurotrophic Factor
    • HCNTF
    • MGC41235
    • OTTHUMP00000174731
    • OTTMUSP00000029848
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100758

  • Representative standard curve using ab100758

实验方案

文献

ab100758 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abreview for CNTF Rat ELISA Kit

Average Average 3/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (blood)
Specification: blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: rat serum
purified CNTF
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Wanted to use the kit to measure CNTF in mouse serum, but it did not work for the mouse samples. Mouse serum was used in multiple amounts, but the plate wells containing the mouse samples were no different than background. The kit worked well with serial dilutions of my positive control (rat serum), though. The kit is otherwise easy to use.
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提交于 Dec 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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