大鼠Ceruloplasmin ELISA试剂盒(CERP) (ab108820)

概述

  • 产品名称大鼠Ceruloplasmin ELISA试剂盒(CERP)
    参阅全部 Ceruloplasmin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.4%
    批次间
    样品 n Mean SD CV%
    Overall 7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.7 µg/ml
  • 范围
    0.78 µg/ml - 50 µg/ml
  • 复检

    96 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Ceruloplasmin (CERP) Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in plasma and serum.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Ceruloplasmin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Ceruloplasmin captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 序列相似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Rat Ceruloplasmin ELISA Kit (CERP) 图像

  • Representative Standard Curve using ab108820.

实验方案

Rat Ceruloplasmin ELISA Kit (CERP) (ab108820)参考文献

ab108820 has not yet been referenced specifically in any publications.

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