大鼠Ceruloplasmin ELISA试剂盒(CERP) (ab108819)

概述

  • 产品名称大鼠Ceruloplasmin ELISA试剂盒(CERP)
    参阅全部 Ceruloplasmin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.4%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.3 ng/ml
  • 范围
    0.31 ng/ml - 20 ng/ml
  • 复检

    96 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Ceruloplasmin (CERP) rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in urine and cell culture supernatants.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • 序列相似性Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat Ceruloplasmin ELISA Kit (CERP) 图像

  • Representative Standard Curve using ab108819.

实验方案

Rat Ceruloplasmin ELISA Kit (CERP) (ab108819)参考文献

ab108819 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and interest in our products.

Your customer can use the kit (ab108819) to determine cell lysate ceruloplasmin level, though it is advised to perform a serial dilution of the sample to determine what is the most opti...

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Thank you for your enquiry.

This kit has been tested on cell culture supernatant and urine as samples - but not on serum.

I would advise you to take a look at the datasheet and the Protocol Booklet for further information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"