概述

  • 产品名称
    大鼠C Reactive蛋白ELISA试剂盒(PTX1)
    参阅全部 C Reactive Protein 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.8%
    批次间
    样品 n Mean SD CV%
    Overall 9.6%
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.7 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 回收率

    95 %

  • 检测时间
    3h 30m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma, serum, and cell culture supernatants.

    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab108827.

实验方案

文献

This product has been referenced in:
  • Al-Rasheed NM  et al. Simvastatin Ameliorates Diabetic Cardiomyopathy by Attenuating Oxidative Stress and Inflammation in Rats. Oxid Med Cell Longev 2017:1092015 (2017). Read more (PubMed: 29138670) »
  • Yu CO  et al. The characterization of a full-thickness excision open foot wound model in n5-streptozotocin (STZ)-induced type 2 diabetic rats that mimics diabetic foot ulcer in terms of reduced blood circulation, higher C-reactive protein, elevated inflammation, and reduced cell proliferation. Exp Anim 66:259-269 (2017). Read more (PubMed: 28413186) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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