大鼠C Reactive Protein ELISA试剂盒(PTX1) (ab108827)

概述

  • 产品名称大鼠C Reactive Protein ELISA试剂盒(PTX1)
    参阅全部 C Reactive Protein 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.7 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    96 %

  • 检测时间
    3h 30m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s C Reactive Protein (PTX1) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein in urine, plasma, serum, and cell culture supernatants.

    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 组织特异性Found in plasma.
  • 序列相似性Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Pentraxin 1, short
    • C reactive protein
    • C reactive protein pentraxin related
    • C-reactive protein(1-205)
    • CRP
    • CRP_HUMAN
    • MGC88244
    • Pentraxin 1
    • PTX 1
    • PTX1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Rat C Reactive Protein ELISA Kit (PTX1) 图像

  • Representative Standard Curve using ab108827.

实验方案

Rat C Reactive Protein ELISA Kit (PTX1) (ab108827)参考文献

This product has been referenced in:
  • Xu J  et al. Optimized rapeseed oils rich in endogenous micronutrients ameliorate risk factors of atherosclerosis in high fat diet fed rats. Lipids Health Dis 13:166 (2014). Rat . Read more (PubMed: 25358951) »

See 1 Publication for this product

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