大鼠BNP 45 ELISA试剂盒(ab108816)
Key features and details
- Sensitivity: 7.9 ng/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
-
产品名称
大鼠BNP 45 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.9% 批次间 样品 n Mean SD CV% Overall 7.1% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 7.9 ng/ml -
范围
15.625 pg/ml - 1000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.
A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
说明
There is significant cross-reactivity between BNP32 and BNP45.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Rat BNP 45 Antibody 1 x 90µl BNP 45 Microplate (12 x 8 well strips) 1 unit BNP 45 Standard (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
-
功能
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
组织特异性
Brain and also in atria, but at much lower levels than ANP. -
序列相似性
Belongs to the natriuretic peptide family. -
细胞定位
Secreted. - Information by UniProt
-
别名
- ANFB_HUMAN
- BNP(1-32)
- BNP(5-29)
see all -
数据库链接
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (23)
ab108816 被引用在 23 文献中.
- Xiong Y et al. Regulation of SARS CoV-2 host factors in the kidney and heart in rats with 5/6 nephrectomy-effects of salt, ARB, DPP4 inhibitor and SGLT2 blocker. BMC Nephrol 23:117 (2022). PubMed: 35331159
- Wang Z et al. NBP Relieves Cardiac Injury and Reduce Oxidative Stress and Cell Apoptosis in Heart Failure Mice by Activating Nrf2/HO-1/Ca2+-SERCA2a Axis. Evid Based Complement Alternat Med 2022:7464893 (2022). PubMed: 36452141
- Richards C et al. Characterisation of cardiac health in the reduced uterine perfusion pressure model and a 3D cardiac spheroid model, of preeclampsia. Biol Sex Differ 12:31 (2021). PubMed: 33879252
- Shi H et al. Velvet Antler Ameliorates Cardiac Function by Restoring Sarcoplasmic Reticulum Ca2+-ATPase Activity in Rats With Heart Failure After Myocardial Infarction. Front Pharmacol 12:621194 (2021). PubMed: 33995020
- Rocca C et al. The chromogranin A1-373 fragment reveals how a single change in the protein sequence exerts strong cardioregulatory effects by engaging neuropilin-1. Acta Physiol (Oxf) 231:e13570 (2021). PubMed: 33073482