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|Cell culture supernatant||132.1||123% - 142%|
|Serum||111.7||102% - 121%|
|Plasma||97.02||70% - 126%|
Abcam’s betaNGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat betaNGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Rat betaNGF coated on a 96-well plate. Standards and samples are pipetted into the wells and betaNGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat betaNGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of betaNGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|组件||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|betaNGF Microplate (12 strips x 8 wells)||1 unit|
|betaNGF Standard Rat (recombinant)||2 vials|
|Biotinylated anti-rat beta-NGF (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100757 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|