大鼠Albumin ELISA试剂盒(ab235642)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 94.64 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 9 3.7% 批次间 样品 n Mean SD CV% Serum 3 3.3% -
样品类型
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
94.64 pg/ml -
范围
312.5 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 113 111% - 116% Serum 109 108% - 111% Hep Plasma 104 102% - 106% EDTA Plasma 107 106% - 108% Cit plasma 113 108% - 117% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Rat
不与反应: Cow, Rhesus monkey -
产品概述
Rat Albumin ELISA Kit (ab235642) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Albumin with 94.64 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 10X Rat Albumin Capture Antibody 1 x 600µl 1 x 6000µl 10X Rat Albumin Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 5BR 1 x 6ml 10 x 6ml Plate Seals 1 unit 1 x 10 units Rat Albumin Lyophilized Recombinant Protein 2 vials 2 x 10 vials Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 24186 Rat
- SwissProt: P02770 Rat
- Unigene: 202968 Rat
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The Albumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:2 million, plasma (heparin) 1:8 million, plasma (EDTA) 1:4 million, and plasma (citrate) 1:4 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration in the neat samples was determined to be 32 mg/mL in serum, 29 mg/mL in plasma (heparin), 26 mg/mL in plasma (EDTA), and 23 mg/mL in plasma (citrate).
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The concentrations of Albumin were measured in duplicate and interpolated from the Albumin standard curve and corrected for sample dilution. Undiluted samples are 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration in the neat sample was determined to be 18 µg/mL.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab235642 被引用在 3 文献中.
- Karwelat D et al. A rodent thyroid-liver chip to capture thyroid toxicity on organ function level. ALTEX 40:83-102 (2023). PubMed: 35791291
- Xiong Y et al. Regulation of SARS CoV-2 host factors in the kidney and heart in rats with 5/6 nephrectomy-effects of salt, ARB, DPP4 inhibitor and SGLT2 blocker. BMC Nephrol 23:117 (2022). PubMed: 35331159
- Alomari G et al. Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin-induced type 1 diabetes in rats. IET Nanobiotechnol 15:473-483 (2021). PubMed: 34694755