概述

  • 产品名称
    大鼠Albumin ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.1%
    批次间
    样品 n Mean SD CV%
    Overall 9.5%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Competitive
  • 灵敏度
    = 0.7 µg/ml
  • 范围
    0.78 µg/ml - 50 µg/ml
  • 复检

    98 %

  • 检测时间
    2h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Rat
  • 产品概述

    Abcam’s Albumin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Albumin in plasma and serum.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

图片

  • Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108790.

实验方案

文献

This product has been referenced in:
  • Arellano-Buendía AS  et al. Urinary excretion of neutrophil gelatinase-associated lipocalin in diabetic rats. Oxid Med Cell Longev 2014:961326 (2014). Read more (PubMed: 25243053) »

See 1 Publication for this product

客户评价及客户问答



I can confirm that 25ul sample per well is used:

The sample is plasma collected using one-tenth volume of 0.1 M sodium citrate as an anticoagulant. Centrifuge samples at 2000 x g for 10 minutes and assay. Dilute samples 1:8000 into ...

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Thank you for contacting us.

Unfortunately we only sell the kits as complete units. These come in from the lab pre-assembled and there is no way for us to split apart kits to sell separately.

I apologize that this information coul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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