Anti-Rat Albumin抗体(FITC) (ab53435)

概述

  • 产品名称
    Anti-Rat Albumin抗体(FITC)
    参阅全部 Rat Albumin 一抗
  • 描述
    羊多克隆抗体to大鼠Albumin (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: IHC-Fr, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Cow
  • 免疫原

    Full length native protein (purified) (Rat)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯化说明
    Antisera to rat albumin were raised by repeated immunisations of sheep with highly purified antigen. The purified IgG was prepared by affinity chromatography.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 19023867
ELISA 1/1000 - 1/25000.
ICC/IF 1/10 - 1/50.
IHC-P Use at an assay dependent concentration. PubMed: 24303036

靶标

  • 功能
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性
    Plasma.
  • 序列相似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alb 1 antibody
    • alb antibody
    • ALBU_RAT antibody
    • Albumin antibody
    • Serum albumin antibody
    see all

Anti-Rat Albumin antibody (FITC) (ab53435)参考文献

This product has been referenced in:
  • Yan T  et al. Niaspan attenuates the adverse effects of bone marrow stromal cell treatment of stroke in type one diabetic rats. PLoS One 8:e81199 (2013). IHC-P ; Rat . Read more (PubMed: 24303036) »
  • He Z  et al. Ischemia-Induced Increase in Microvascular Phosphodiesterase 4D Expression in Rat Hippocampus Associated with Blood Brain Barrier Permeability: Effect of Age. ACS Chem Neurosci 3:428-32 (2012). IHC ; Rat . Read more (PubMed: 22860212) »

See all 4 Publications for this product

Product Wall

Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en est...

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Gracias por tu respuesta.

Como bien indicas, ab53435 solo se ha testado en secciones congeladas, no tengo constancia de que funcione en parafina. De todas maneras, he contactado con mis compañeros del laboratorio para que me lo confirm...

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Espero que no te importe que te conteste en castellano, ya que recientemente hemos inaugurado el Servicio Técnico en español. Si prefirieses seguir contactándonos en ingles, no dudes en comunicármelo.

Si lo que nec...

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Thank you for contacting us.

Indeed, I am happy to let you know that we have anti-rat albumin antibodies (unconjugated and conjugated) available:
ab106582:Click here (or use the following: http://www.abcam.com/index.html?datasheet=10658...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Frozen Liver Tissue Sections)
Specification
Frozen Liver Tissue Sections
Fixative
Methanol
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

提交于 Mar 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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