概述

  • 产品名称Anti-RASSF1a抗体
    参阅全部 RASSF1a 一抗
  • 描述
    兔多克隆抗体to RASSF1a
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 50 and 334 of Human RASSF1a (NP_733832).

  • 阳性对照
    • A431 and HeLaS3 cell lines. 293T whole cell lysate. HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab97749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • 组织特异性Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • 序列相似性Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.
  • 细胞定位Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ras association domain containing protein 1 antibody
    • Ras association domain family 1 antibody
    • Ras association domain family protein 1 antibody
    • Ras association domain-containing protein 1 antibody
    • RASF1_HUMAN antibody
    • RASSF 1a antibody
    • Rassf1 antibody
    • RASSF1A antibody
    • RASSF1A isoform antibody
    • Tumor suppressor protein RDA32 antibody
    see all

Anti-RASSF1a antibody 图像

  • Anti-RASSF1a antibody (ab97749) at 1/500 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 39 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97749 at 1/100 dilution. Lower image is merged with DNA probe.

Anti-RASSF1a antibody (ab97749)参考文献

This product has been referenced in:
  • Bräuer-Hartmann D  et al. PML/RARa-Regulated miR-181a/b Cluster Targets the Tumor Suppressor RASSF1A in Acute Promyelocytic Leukemia. Cancer Res 75:3411-24 (2015). Read more (PubMed: 26041820) »

See 1 Publication for this product

Product Wall

Thanks for your enquiry.
We do not have a specific protocol for cell extraction when preparing samples to western blot RASSF1a. We do have a good general WB protocol which you might find useful at http://www.abcam.com/index.html?pageconfig=resourc...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"