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Recombinant human RASSF1A purified from E. coli.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab23950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
|ICC/IF||Use at an assay dependent concentration. Use at an assay dependent dilution. Use a biotinylated secondary antibody and Texas-red conjugated streptavidin. Use 4% paraformaldehyde as fixative for 20min and permeabilize in 0.4% Triton X-100.|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"