概述

  • 产品名称Anti-RASGRP2抗体
    参阅全部 RASGRP2 一抗
  • 描述
    兔多克隆抗体to RASGRP2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 35-83 (VRMFLMMHPW YIPSSQLAAK LLHIYQQSRK DNSNSLQVKT CHLVRYWISA) of Human RASGRP2 (NP_722541)

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab94865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
  • 组织特异性Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
  • 序列相似性Belongs to the RASGRP family.
    Contains 2 EF-hand domains.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • 发展阶段Expressed in fetal brain, lung, liver and kidney.
  • 结构域The N-terminal Ras-GEF domain mediates association with F-actin.
  • 翻译后修饰Isoform 2 is palmitoylated and myristoylated while isoform 1.
  • 细胞定位Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium and DAG-regulated antibody
    • Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
    • Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
    • CalDAG-GEFI antibody
    • Cdc25 like protein antibody
    • Cdc25-like protein antibody
    • F25B3.3 kinase like protein antibody
    • F25B3.3 kinase-like protein antibody
    • GRP2_HUMAN antibody
    • HCDC25L antibody
    • RAS guanyl releasing protein 2 antibody
    • RAS guanyl-releasing protein 2 antibody
    • Rasgrp2 antibody
    see all

Anti-RASGRP2 antibody 图像

  • Anti-RASGRP2 antibody (ab94865) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa

Anti-RASGRP2 antibody (ab94865)参考文献

ab94865 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab94865.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"