概述

  • 产品名称Anti-RASGRP 4抗体
    参阅全部 RASGRP 4 一抗
  • 描述
    兔多克隆抗体to RASGRP 4
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 108 - 170 (KATGDTQELR RLQICHLVRY WLMRHPEVMH QDPQLEEVIG RFWATVAREG NSAQRRLGDS SDL) of Human RASGRP 4 (NP_733749).

  • 阳性对照
    • WB: A431, H1299, HeLa and Raji cell lysates IHC-P: SW480 (Human colon adenocarcinoma) tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.

靶标

  • 功能Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
  • 组织特异性Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
  • 序列相似性Belongs to the RASGRP family.
    Contains 1 EF-hand domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • 发展阶段Expressed early during development. Detected in all fetal tissues tested.
  • 结构域The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.
  • 细胞定位Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GRP4_HUMAN antibody
    • Guanyl nucleotide releasing protein 4 antibody
    • RAS guanyl releasing protein 4 antibody
    • RAS guanyl-releasing protein 4 antibody
    • RASGRP4 antibody
    see all

Anti-RASGRP 4 antibody 图像

  • ICC/IF image of ab96293 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96293 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RASGRP 4 antibody (ab96293) at 1/1000 dilution + Raji whole cell lysates at 30 µg

    Predicted band size : 75 kDa
    7.5% SDS PAGE
  • ab96293, at a 1/100 dilution, staining RASGRP 4 in paraffin embedded SW480 (Human colon adenocarcinoma) tissue by Immunohistochemical analysis.

Anti-RASGRP 4 antibody (ab96293)参考文献

ab96293 has not yet been referenced specifically in any publications.

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