概述

  • 产品名称Anti-RASGRP 4抗体
    参阅全部 RASGRP 4 一抗
  • 描述
    小鼠单克隆抗体to RASGRP 4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: CGLCCHKHCR DQVKVECKKR PGAKGDAGPP GAPVPSTPAP HASCGSEENH SYTLSLEPET GCQLRHAWTQ TESPHPSWET DTVPCPVMDP PSTASSKLDS , corresponding to amino acids 574-674 of Human RASGRP 4

性能

应用

Our Abpromise guarantee covers the use of ab57978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
    • 组织特异性Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
    • 序列相似性Belongs to the RASGRP family.
      Contains 1 EF-hand domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • 发展阶段Expressed early during development. Detected in all fetal tissues tested.
    • 结构域The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.
    • 细胞定位Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GRP4_HUMAN antibody
      • Guanyl nucleotide releasing protein 4 antibody
      • RAS guanyl releasing protein 4 antibody
      • RAS guanyl-releasing protein 4 antibody
      • RASGRP4 antibody
      see all

    Anti-RASGRP 4 antibody 图像

    • RASGRP 4 antibody (ab57978) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab57978 RASGRP 4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-RASGRP 4 antibody (ab57978)参考文献

    ab57978 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab57978.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"