The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: limiting dilution: 1/32000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
An additional band of unknown identity was also consistently observed at 21kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Probable inhibitory regulator of the Ras-cyclic AMP pathway.
Highly expressed in thyroid and adrenal medulla, lower expression in brain, spinal cord and trachea.