The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC: Use at a concentration of 10 µg/ml. This concentration is recommended to detect the protein in ethanol:acetic acid-fixed [50:50] A431 cells.
IP: 1µg is recommended to precipitate the protein from 250 µg of an A431 cell lysate.
WB: Use at a concentration of 1 µg/ml, with 10 µg of an A431 cell lysate. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).
Fernández-Medarde A et al. Laser microdissection and microarray analysis of the hippocampus of Ras-GRF1 knockout mice reveals gene expression changes affecting signal transduction pathways related to memory and learning. Neuroscience146:272-85 (2007).
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