RabMAb

Anti-RASA1 (phospho Y460)抗体[EPR2620(2)] (ab109465)

概述

  • 产品名称Anti-RASA1 (phospho Y460)抗体[EPR2620(2)]
    参阅全部 RASA1 一抗
  • 描述
    兔单克隆抗体[EPR2620(2)] to RASA1 (phospho Y460)
  • 特异性ab109465 only detects GAP phosphorylated at Tyrosine 460.
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Tyrosine 460 of Human GAP.

  • 阳性对照
    • 293T cell lysates; 293T cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab109465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.
    • 组织特异性In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
    • 疾病相关Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
      Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
      Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.
    • 序列相似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 Ras-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻译后修饰The N-terminus is blocked.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CM AVM antibody
      • CMAVM antibody
      • DKFZp434N071 antibody
      • GAP antibody
      • GTPase activating protein antibody
      • GTPase-activating protein antibody
      • OTTHUMP00000222390 antibody
      • OTTHUMP00000222391 antibody
      • OTTHUMP00000222392 antibody
      • OTTHUMP00000222393 antibody
      • p120GAP antibody
      • p120RASGAP antibody
      • PKWS antibody
      • Ras GTPase-activating protein 1 antibody
      • RAS p21 protein activator (GTPase activating protein) 1 antibody
      • Ras p21 protein activator antibody
      • RASA antibody
      • RASA1 antibody
      • RASA1_HUMAN antibody
      • RasGAP antibody
      • Triphosphatase activating protein antibody
      see all

    Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] 图像

    • All lanes : Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465) at 1/1000 dilution

      Lane 1 : 293T cell lysates, untreated
      Lane 2 : 293T cell lysates treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 116 kDa
    • ab109465 at 1/100 dilution staining GAP in 293T cells by Immunofluorescence.

    Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)参考文献

    ab109465 has not yet been referenced specifically in any publications.

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