概述

  • 产品名称Anti-Ras抗体[EPR3255]
    参阅全部 Ras 一抗
  • 描述
    兔单克隆抗体[EPR3255] to Ras
  • 特异性ab108602 also detects H-Ras and N-Ras.
  • 经测试应用适用于: WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminus of Human K-Ras.

  • 阳性对照
    • WB: 293T and SH-SY5Y cell lysates.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • 疾病相关Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
      Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
      Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
      Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
      Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
      Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
    • 序列相似性Belongs to the small GTPase superfamily. Ras family.
    • 翻译后修饰Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
    • 细胞定位Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C-BAS/HAS antibody
      • c-H-ras antibody
      • C-HA-RAS1 antibody
      • CTLO antibody
      • GTPase HRas antibody
      • GTPase KRas antibody
      • GTPase NRas antibody
      • H-Ras-1 antibody
      • H-RASIDX antibody
      • Ha-Ras antibody
      • HAMSV antibody
      • HRAS antibody
      • HRAS1 antibody
      • K RAS2A antibody
      • K RAS2B antibody
      • K RAS4A antibody
      • K RAS4B antibody
      • K-RAS antibody
      • KRAS antibody
      • KRAS1 antibody
      • KRAS2 antibody
      • N-RAS antibody
      • N-terminally processed antibody
      • NRAS antibody
      • NRAS1 antibody
      • p21ras antibody
      • RASH_HUMAN antibody
      • RASH1 antibody
      • RASK2 antibody
      • Transforming protein p21 antibody
      • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
      • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
      • v ras neuroblastoma RAS viral oncogene homolog antibody
      see all

    Anti-Ras antibody [EPR3255] 图像

    • All lanes : Anti-Ras antibody [EPR3255] (ab108602) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa

    Anti-Ras antibody [EPR3255] (ab108602)参考文献

    This product has been referenced in:
    • Li W  et al. The association between expressions of Ras and CD68 in the angiogenesis of breast cancers. Cancer Cell Int 15:17 (2015). IHC-P ; Human . Read more (PubMed: 25685069) »
    • Sos ML  et al. Oncogene mimicry as a mechanism of primary resistance to BRAF inhibitors. Cell Rep 8:1037-48 (2014). IP, WB . Read more (PubMed: 25127139) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-20% SDS-PAGE 35mA 40mins)
    Sample Human Cell lysate - whole cell (Melanoma)
    Specification Melanoma
    Blocking step Fish Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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    提交于 Jul 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"