Anti-Ras抗体[EP1125Y] (ab52939)


  • 产品名称Anti-Ras抗体[EP1125Y]
    参阅全部 Ras 一抗
  • 描述
    兔单克隆抗体[EP1125Y] to Ras
  • 特异性ab52939 is predicted to react with H-Ras, N-Ras and K-Ras.
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminal region of human Ras.

  • 阳性对照
    • C6 cell lysate and permeabilized PC-12 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


Our Abpromise guarantee covers the use of ab52939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP 1/30.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • 疾病相关Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
      Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
      Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
      Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
      Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
      Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
    • 序列相似性Belongs to the small GTPase superfamily. Ras family.
    • 翻译后修饰Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
    • 细胞定位Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C-BAS/HAS antibody
      • c-H-ras antibody
      • C-HA-RAS1 antibody
      • CTLO antibody
      • GTPase HRas antibody
      • GTPase KRas antibody
      • GTPase NRas antibody
      • H-Ras-1 antibody
      • H-RASIDX antibody
      • Ha-Ras antibody
      • HAMSV antibody
      • HRAS antibody
      • HRAS1 antibody
      • K RAS2A antibody
      • K RAS2B antibody
      • K RAS4A antibody
      • K RAS4B antibody
      • K-RAS antibody
      • KRAS antibody
      • KRAS1 antibody
      • KRAS2 antibody
      • N-RAS antibody
      • N-terminally processed antibody
      • NRAS antibody
      • NRAS1 antibody
      • p21ras antibody
      • RASH_HUMAN antibody
      • RASH1 antibody
      • RASK2 antibody
      • Transforming protein p21 antibody
      • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
      • v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
      • v ras neuroblastoma RAS viral oncogene homolog antibody
      see all

    Anti-Ras antibody [EP1125Y] 图像

    • Anti-Ras antibody [EP1125Y] (ab52939) at 1/500000 dilution + C6 cell lysate at 10 µg/ml

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 cells by flow cytometry (red). Rabbit IgG negative control (green).
    • Anti-Ras antibody [EP1125Y] (ab52939) at 1/500 dilution + Human Ras full length protein (ab56526) at 0.01 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    Anti-Ras antibody [EP1125Y] (ab52939)参考文献

    This product has been referenced in:

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Arachnoidal cells)
    Gel Running Conditions Reduced Denaturing (12%)
    Loading amount 15 µg
    Specification Arachnoidal cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    提交于 Mar 02 2016

    Thank you for kindly confirming these details. I am sorry these antibodies have not worked for this customer.

    As requested, we are pleased to arrange the alternative free of charge replacements. I can confirm that 1 vial of ab87980 and 1 via...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer hashad difficulty obtaining satisfactory results from the free of charge replacement vials.

    The details you have kindly provided will...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549)
    Loading amount 50 µg
    Specification A549
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 Sep 17 2010

    Thank you for your enquiry. I have obtained some further information on the flow cytometry protocol used to test this antibody. 2% paraformaldehyde was used for fixation: Solutions and Reagents: 1. 1X PBS 2. Blocking buffer 0.5% BSA in 1X PBS ...

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