概述

  • 产品名称Anti-RARRES1抗体
    参阅全部 RARRES1 一抗
  • 描述
    小鼠单克隆抗体to RARRES1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: EMTTQVSHYY LAQLTSVRQW KTNDDTIDFD YTVLLHELST QEIIPCRIHL VWYPGKPLKV KYHCQELQTP EEASGTEEGS AVVPTELSNF , corresponding to amino acids 205-295 of Human RARRES1 with a 26kDa proprietary tag (NP_996846)

  • 阳性对照
    • HL60 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab92884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Belongs to the protease inhibitor I47 (latexin) family.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5430417P09Rik antibody
      • AI662122 antibody
      • RAR-responsive protein TIG1 antibody
      • RARRES1 antibody
      • Retinoic acid receptor responder (tazarotene induced) 1 antibody
      • Retinoic acid receptor responder protein 1 antibody
      • Tazarotene induced gene 1 protein antibody
      • Tazarotene-induced gene 1 protein antibody
      • TIG1 antibody
      • TIG1_HUMAN antibody
      see all

    Anti-RARRES1 antibody 图像

    • Anti-RARRES1 antibody (ab92884) at 5 µg/ml + recombinant immunogen at 0.2 µg

      Secondary
      Goat anti-Mouse IgG HRP at 1/5000 dilution

      Predicted band size : 33 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    • Anti-RARRES1 antibody (ab92884) at 5 µg/ml + HL60 cell lysate at 25 µg

      Secondary
      Goat anti-Mouse IgG HRP at 1/2500 dilution

      Predicted band size : 33 kDa

    Anti-RARRES1 antibody (ab92884)参考文献

    This product has been referenced in:
    • Sahab ZJ  et al. Tumor suppressor RARRES1 interacts with cytoplasmic carboxypeptidase AGBL2 to regulate the a-tubulin tyrosination cycle. Cancer Res 71:1219-28 (2011). WB ; Human . Read more (PubMed: 21303978) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Hydrogen Peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample Human Tissue sections (Placenta)
    Specification Placenta
    Permeabilization Yes - Tris
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 06 2013

    Vielen Dank für Ihre Hilfe.


    Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge zu Veränderung Ihres Protokolls machen:

    1. Messen Sie die Proteinkonzentration. Bei einer Probe, in der das Protein...

    Read More

    Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

    Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

    Um Ihr Problem zu lösen, möc...

    Read More

    Vielen Dank für Ihren Anruf.

    Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

    Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogen...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"