Anti-Raptor抗体(ab62454)
Key features and details
- Rabbit polyclonal to Raptor
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Raptor抗体
参阅全部 Raptor 一抗 -
描述
兔多克隆抗体to Raptor -
宿主
Rabbit -
特异性
This antibody reacts with Raptor. Raptor has multiple isoforms that may also be recognized by this antibody. -
经测试应用
适用于: WB, ICC/IF, ICCmore details -
种属反应性
与反应: Mouse -
免疫原
A 15 amino acid synthetic peptide from the central region of human Raptor
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阳性对照
- L1210 cell lysate and L1210 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62454于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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ICC |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
ICC
Use a concentration of 1 µg/ml. |
靶标
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功能
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. -
组织特异性
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. -
序列相似性
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats. -
细胞定位
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability. - Information by UniProt
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数据库链接
- Entrez Gene: 74370 Mouse
- SwissProt: Q8K4Q0 Mouse
- Unigene: 209933 Mouse
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别名
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
see all
图片
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Lane 1 : Anti-Raptor antibody (ab62454) at 2 µg/ml
Lane 2 : Anti-Raptor antibody (ab62454) at 4 µg/ml
All lanes : L1210 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 149 kDa
Observed band size: 149 kDa -
Immunocytochemical staining of L1210 cells using Raptor antibody at 1µg/ml.
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Immunofluorescence of Raptor in L1210 cells using ab62454 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62454 尚未被引用在任何文献中。